• Gold standard for His-tag protein purification
• Our Ni-NTA resin has the highest tolerance for reducing agents such as DTT (up to 10 mM) or 2-ME (up to 20 mM)
– Some Ni-IMAC resins tend to turn brown after applying reducing agents
• Superior stability to EDTA (up to 1.5 mM)
• Very high reproducibility
– Matrix consists of 7.5% cross-linked agarose
– Extremely homogenous in size
FEATURES
Specificity: His-Tag
Matrix: 6% cross-linked agarose (IDA) or 7.5% cross-linked agarose (Ni-NTA)
Binding capacity: 10 mg/ml (IDA), 70 mg/ml (Ni-NTA)
Bead size range: 45-165 μm (IDA), 32-60 μm (Ni-NTA)
Maximum pressure: 42 psi (IDA), 72 psi (Ni-NTA)
Stability/storage: pH 2-12 (IDA), pH 2-14 (Ni-NTA)
Equilibration buffer (short-term)
20% Ethanol at 2-8°C (long-term)
No data available.