The Anatrace Proteus 1-Step Batch Mini Spin Columns are ideal for small scale protein purifications such as those required for expression trials, solubility determination tests, screening, tittering, and scouting studies. If you’re working with handling bio-hazardous samples and samples from infectious sources the device is self-contained and easy to handle and dispose of.
Developed as a substitute to gravity-driven filtration, the Proteus 1-Step Batch Spin Columns from Anatrace have the advantage of not requiring constant attention while supernatant filters through the column and resin.
The innovative Proteus 1-Step Batch Mini Spin Columns allow you to:
1. Batch mix your unfiltered supernatant sample with the resin of your choice.
2. The SelfSeal™ membrane technology seals your resin and supernatant in the incubation chamber.
3. To separate your protein from the supernatant spin the column in a centrifuge. The centrifuge action opens up the SelfSeal™ membrane pores allowing the supernatant to run through leaving your protein bound to the resin.
4. To collect your protein, wash and elute your purified and concentrated protein in a small volume.
The system has no resin carry-over into subsequent downstream analyses, no messy transfer of samples/resin slurries and negligible hold-up volume.
The 1-Step Batch Mini Spin Columns can accommodate sample volumes up to 0.6 ml.
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