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This month we are celebrating all that has been accomplished in 2017. Higher standards in many areas of structural biology have been set, from crystallizing the LRRK2 protein on the International Space Station, to determining the structures of GPR40, TRPV6, and the GLP-1R:peptide complex...not to mention the incredible progress still being made in the field of Cryo-EM.
At Anatrace, our year has been equally exciting, as we’ve added Molecular Dimensions to our roster – allowing us to further support the full gene-to-structure pipeline for both membrane and soluble proteins.
We thank you for your business with two special offers. To learn more, click here. |
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Anatrace is pleased to announce the recent characterization of the alkyl glucoside n-dodecyl-ß-melibioside (ß-DDMB) by Chuck Sanders and his lab at Vanderbilt University. This new detergent differs from traditional DDM in that it contains the disaccharide melibiose instead of maltose as the head group, giving it a slightly higher CMC and smaller micelles. The size of DDMB’s micelles doesn’t change with detergent concentration, temperature, pH, or ionic strength, like it does with DDM. For more information please click here.
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The number of protein structures determined by cryo-electron microscopy (Cryo-EM) has increased in just one year by an impressive 46%. Membrane proteins determined by Cryo-EM has grown by an incredible 80% growth. We have compiled a table of the most recent structures for reference, along with the detergents used in their determination. To evaluate which Cryo-EM detergents and tools can help you in your research, please click here. |
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Researchers sent the Anatrace Microlytic Crystal Formers to the International Space Station to perform microgravity crystallization experiments of the LRRK2 protein. LRRK2 is one of the promising drug targets for the treatment of Parkinson’s disease because mutations in the gene encoding this protein are among the most common genetic causes.
To find out more about this outer space effort, click here. And to learn how you can support efforts to find a cure for Parkinson’s, visit michaeljfox.org. |
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Anatrace's 30 years in the lab has led to the most commonly used, top-performing, and competitively priced purification resins on the market.
For those involved in affinity purification of poly-histidine tagged proteins through IMAC, we offer our Super-Nickel and Super-Cobalt resins. Our Super-GLU glutathione coupled agarose resin is also a solid performer for those purifying GST-tagged proteins. For immunoglobulin purification, we have our immobilized Protein A and Protein G resins. And for those looking to purify nucleic acid binding proteins, as well as enzymes, hormone receptors, and extracellular matrix proteins, we now offer our new Super Heparin High-Performance Resin. To make things even sweeter, until September 30th, we're offering 10% off your order of purification resins and HiFliQ FPLC Columns.
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The feature this month is the recent publication of two additional structures of the GPR40 protein which identify a novel binding site for full allosteric agonists by Merck Research Laboratories. It furthers the investigation done previously by Amgen and Takeda Pharmaceuticals on the GPR40 receptor. Anatrace was involved in the discovery process with our DDM/CHS pre-made solution and LMNG detergent. And to better understand the progress being made to combat this disease, as well as how Anatrace can help your lab discover innovative solutions for this and much more, click here. |
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We are pleased to introduce our new Biotinylated Amphipol A8-35. We’re giving you another way to help you quickly determine the function of your membrane protein. With a polymer formulation that allows for the immobilization of membrane proteins onto solid supports, our Biotinylated Amphipol A8-35 allows you to do binding assays you typically can’t in detergents without modifying the protein, such as SPR, ELISA and Microscopy. Plus, reconstitution into amphipol is straightforward and simple. |
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Today's labs are finding that fluorinated surfactants are an effective alternative to the use of detergents when working with membrane proteins. Whether it’s improving membrane protein stability, aiding the vitrification process in Cryo-EM, or as an additive in protein crystallization,
fluorinated surfactants have a number of useful applications for protein biochemistry. |
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This month we are featuring the recent identification of two antibodies with RSV neutralizing activity. Learn about this exciting publication in Nature Communications by the labs of Xavier Saelens at Ghent University and VIB in Belgium and Jason McLellan at Dartmouth College describing the identification of two single-domain antibodies that bind to the protein on the virus that’s essential for infection. A significant part of this study involved elucidating how these antibodies interact with this viral protein. Using the free-interface diffusion method in our Microlytic Crystal Former, co-crystals were obtained with dramatically improved diffraction as compared to those grown using vapor diffusion. |
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Anatrace now fully supports the entire gene-to-structure pipeline for both soluble and membrane protein targets with the recent addition of the Molecular Dimensions product portfolio to their offering. Molecular Dimensions is the market leader in protein crystallization. The greatest minds in crystallography will now be working together to develop new ideas, new products, and new solutions to continually raise the bar. All of these products will be available through a single source, making future ordering and logistics a breeze. |
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2016 was an outrageously exciting year for membrane protein research. Nearly 90 unique structures were deposited last year – the most ever. We wanted to share with you our updated data originally created to summarize the detergents most frequently used in 2015's breakthrough studies. Using the current list of unique structures published in 2016 from Steve White's "Membrane Proteins of Known 3D Structure" database, we mined the literature to determine which detergents were most frequently used in membrane protein solubilization, purification, and structure determination experiments. So if you're trying to choose a detergent for your membrane protein solubilization, purification, or structure determination experiments, take a quick look at our updated data. |
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This month we are highlighting the breakthrough study of the determination of the first structure of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) through the use of single-particle electron cryo-microscopy (cryo-EM). Learn about this groundbreaking step forward by scientists Zhe Zhang and Jue Chen from The Rockefeller University. |