Introducing the new and improved Non-Ionic Amphipol (NAPol)

Amphipols (APols) are a class of polymers which can stabilize membrane proteins in a detergent-free, aqueous solution (1). Membrane proteins are first solubilized and purified using detergents (e.g. DDM). APols are then added to the solution and the detergents are removed, resulting in an amphipol reconstituted membrane protein. Further development of chemically different APols, including zwitterionic APols and nonionic amphipols (NAPols), has led to increased levels of stabilizing efficiency, with NAPols being the most stabilizing derivatives.

 

NAPols were first developed at the University of Avignon in the laboratory of Bernard Pucci (2), and further characterized by the laboratory of Jean-Luc Popot at CNRS (3). NAPol consists of repeating units of two glucose molecules linked to a C11 alkyl chain. They are highly soluble in water and self-organise into small compact particles of 6nm in diameter (2). In comparison to APols, NAPols are easier to synthesize in a reproducible manner between batches and they are less heterogeneous at the molecular level.

We are kicking off 2021 continuing to highlight amphipols promoting our newest addition to the Anatrace amphipol family, the Non-Ionic Amphipol (NAPol) introduced in our August 2019 Newsletter, contact us for more information and use coupon code NAPol to get 10% off your next order, now through the end of March 2021!

NAPol.   (N=10-50)

n=10-50

NAPol Key Benefits:

  • New and Improved synthesis methodology: Mercury free conditions
  • Soluble across a wide pH range
  • Compatible with multivalent cations (e.g. Ca2+ / Mg2+) (4)
  • Compatible with a wide range of biophysical and structural techniques:
    • Cell-free synthesis (3)
    • Enzymatic assays
    • NMR (2), Cryo-EM (5-7)
    • SAXS(8)
  • Available in pack sizes of 10mg, 100mg, 250mg and 500mg, (custom sizes available)
  • Ample stock for quick delivery
Available Now!
     

Structure of C. reinhardtii C2S2M2L2-type PSII supercomplex(7)

Structure-of-C.-reinhardtii-C2S2M2L2-type-PSII-supercomplex
 

5.8Å Cryo-EM structure of photosystem II (PSII) Light Harvesting Complex II (LHCII) super complex (C2S2M2L2) with NAPol. Using NAPol during the purification procedure resulted in a dramatic increase in the complete C2S2M2Lsuper-complex (68%) compared to when purified in DDM (7%) (7).

 

References:

  1. Tribet, C., et al. (1996) Proc Natl Acad Sci U S A 93(26), 15047-15050.
  2. Sharma, KS, et al. (2012) Langmuir 28(10), 4625-4639.
  3. Bazzacco, P, et al. (2012) Biochemistry 51(7), 1416-1430.
  4. Jin F, et al (2020) BioRxiv https://doi.org/10.1101/2020.09.28.316307
  5. Bausewein, T, et al. (2017) Cell 170(4), 693-700.
  6. Kubota-Kawai, H, et al. (2019) J Biol Chem 294(12), 4304-4314.
  7. Burton-Smith, RN, et al. (2019) J Biol Chem doi: 10.1074/jbc.RA119.009341.
  8. Solyom, Z, et al (2015) Biophysical Journal 109; 1483-1496