In last month’s newsletter, we highlighted several recent results using Biotinylated Amphipol A8-35, and launched our newest functionalized amphipol, Biotinylated PMAL-C8. To recap, amphipols are a class of polymers which can stabilize membrane proteins in a detergent-free, aqueous solution(1). To reconstitute a membrane protein in to amphipols, first the protein is solubilized and purified using detergents (e.g. DDM). Amphipols are then added to the solution and the detergents are removed, resulting in an amphipol reconstituted membrane protein.
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